Skip to main content
Premium Trial:

Request an Annual Quote

At Beyond Genome, Naylor Identifies 10 Challenges Facing Proteomic Scientists

SAN FRANCISCO, June 23 (GenomeWeb News) - Kicking off the proteomics track at the Beyond Genome conference here today, Stephen Naylor from MIT's CSBi program introduced a number of challenges he sees facing proteomics scientists.

 

The good news, he noted, is that proteomics is getting more attention from more researchers, noting that the majority of talks and papers at this year's ASMS meeting last month involved proteomics. He also assured conference attendees that there have been plenty of advances moving the field forward.

 

But there's some bad news too, he said, including what he called the "graveyard" of embattled or completely failed proteomics companies.

 

In a call for better technology and analytical capabilities, Naylor identified proteomics as the current "technology hole" in the systems biology spectrum. Acknowledging that "there's been some tremendous work done in the past several years" in proteomics, he contended that the incredible complexity of the discipline makes it far more problematic than other systems biology components such as the more established genomics or the longer-lived metabolomics.

 

Naylor's talk started the day for the proteomics part of the conference, and the subsequent talks in that track for the day will be devoted to elucidating each of the 10 challenges he listed. To illustrate just one of the challenges -- issues related to dynamic range -- Naylor demonstrated that because current technologies detect the most abundant proteins, which tend to be the same over and over, studies of protein patterns haven't been able to distinguish between, say, a patient with Alzheimer's and one with colon cancer. He added that while there are differences in patterns between the proteins found, it hasn't yet been proven that those differences stem from biological variation rather than platform variation.

 

Today's talks will address 10 challenges in proteomics, which are: complex mixture analysis; differential proteomics; relative and absolute quantitation; dynamic range; membrane proteins; post-translational modification; throughput; protein arrays and multiplexing; protein expression and production; protein informatics.

 

What's needed to face these issues, Naylor said, include routine analysis; robustness; reproducibility; sensitivity; specificity; selectivity; speed; and capability for stoichiometric quantitation.

The Scan

Genome Sequences Reveal Range Mutations in Induced Pluripotent Stem Cells

Researchers in Nature Genetics detect somatic mutation variation across iPSCs generated from blood or skin fibroblast cell sources, along with selection for BCOR gene mutations.

Researchers Reprogram Plant Roots With Synthetic Genetic Circuit Strategy

Root gene expression was altered with the help of genetic circuits built around a series of synthetic transcriptional regulators in the Nicotiana benthamiana plant in a Science paper.

Infectious Disease Tracking Study Compares Genome Sequencing Approaches

Researchers in BMC Genomics see advantages for capture-based Illumina sequencing and amplicon-based sequencing on the Nanopore instrument, depending on the situation or samples available.

LINE-1 Linked to Premature Aging Conditions

Researchers report in Science Translational Medicine that the accumulation of LINE-1 RNA contributes to premature aging conditions and that symptoms can be improved by targeting them.