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ABRF Seeking Participants for Recombinant Protein Expression Study

NEW YORK (GenomeWeb News) - The Association of Biomolecular Resource Facilities’ Protein Expression Research Group is inviting researchers to participate in its study of recombinant protein expression in E. coli.
 
The group’s goal is to determine the variability of results with expression and purification of a common His-tagged protein. PERG will provide researchers with a plasmid expression construct, information about the construct and encoded cDNA, and instructions regarding how to participate in the study.
 
The study is open to members and non-members of ABRF. Those new to recombinant protein expression will be given a basic protocol on how to do the expression and purification. Experienced scientists are encouraged to use their own protocols. Participants will remain anonymous but be able to see how their results compared to others and what conditions resulted in the greatest yield and purity.
 
The study will remain open until March 31. Preliminary results for data returned early will be presented at ABRF’s conference next month.
 
Further information is available here.

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