In May 2005, Genome Technology celebrated the 20th anniversary of PCR, with an eye on the future of the "gold standard" of DNA amplification. At the time, industry watchers were waiting for Roche's core PCR patents to expire and predicted that enzyme prices would drop — allowing more suppliers to enter the field and the technology to grow even faster. At the same time, researchers were beginning to explore RT-PCR for handling RNA-based samples and Andy Felton, a product manager at Applied Biosystems, predicted that using PCR for microRNA would be the next big wave.

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