➢ Human induced pluripotent stem cell (iPSC) technology has provided unique ways to understand and potentially treat human diseases using cells from individual patients. ➢ iPS cells are amenable to genomic modifications using site-specific gene editing technologies such as CRISPR/Cas9, by which we can correct/ introduce precise disease causing mutations in patient or healthy iPSCs, respectively. ➢ Genome edited iPSCs and their isogenic control (or parental line) offer highly-controlled experimental models for reliable comparison of results.
The goal of this experiment was to utilize the Echo 525 Liquid Handler in assembling five, five-piece assemblies by substituting modular DNA pieces at the nanoliter scale. The Labcyte Echo Liquid Handler enables lower-cost methods and workflows to produce high-quality synthetic DNA constructs.
Before beginning a large animal study, it is critical to confirm that your labeled targeting agent is specific to the intended target within the biological complexity of the whole animal. Understanding the biodistribution and clearance of a labeled targeting agent is critical for meaningful image analysis and interpretation. This guide will provide an overview of how to validate a targeting agent in vivo.