DNA quantification and normalization are required in most genomics workflows, and are usually routinely implemented after the initial nucleic acid purification step. The versatility of the classical absorbance technique still makes it the go-to method for many applications, as the concentration of all DNA and RNA types can be determined using the specific empiric extinction coefficient for each nucleic acid type.
This white paper demonstrates how the Fluent Laboratory Automation Solution offers excellent reproducibility for UV quantification and normalization.
Quantitative PCR (qPCR) is a preferred method for quantification of low amounts of DNA or cDNA due to its high sensitivity, specificity and reproducibility. Consistently high and uniform qPCR efficiencies are crucial for accurate relative quantification of gene expression levels, low copy gene detection, or absolute quantification of NGS libraries.
Genomics is facilitating a deeper understanding of cancer biology and through clinical research, new biomarkers are emerging that pave the way for next-generation therapeutics to help fight cancer in a more personalized way.
In this webinar, Sandip Patel, MD will provide a review of biomarker research and discoveries in the field of Immunotherapy and Garret Hampton, PhD will discuss Illumina’s vision for Oncology and share how genomics will enable broader availability of comprehensive tumor sequencing to explore complex biomarkers and assess tumor mutational burden.