Columbia Researchers’ New SBS Method Combines Sanger with Reversible Termination

In a study, the hybrid approach, which employs both fluorescent dideoxy nucleotide terminators and unlabeled reversible terminators, was able to determine 32 bases of DNA on a chip, reading it with a four-color fluorescence scanner. With further improvements, especially in the sensitivity of fluorescent detection, the authors say the read length could potentially reach more than 700 base pairs.

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