Chromosome-Scale Selective Sweeps and Genomic Diversity in C. elegans
Andersen, Gerke et al., Nature Genetics
Researchers at Princeton University and elsewhere discuss the effects of chromosome-scale selective sweeps on genomic diversity in Caenorhabditis elegans. Taking a high-throughput selective sequencing approach on a collection of 200 wild C. elegans strains, the team found that the nematode's "genome variation is dominated by a set of commonly shared haplotypes on four of its six chromosomes, each spanning many megabases." Further, the team reports on its population genetic modeling experiments, which showed that "this pattern was generated by chromosome-scale selective sweeps that have reduced variation worldwide; at least one of these sweeps probably occurred in the last few hundred years," it writes.
BC Team's Tag-Seq Protocol Reduces Costs and Improves Throughput of LongSAGE, Complements RNA-Seq
The approach modifies protocols that are used for long serial analysis of gene expression, or LongSAGE, for the Illumina Genome Analyzer and can generate two orders of magnitude more data than Sanger-based LongSAGE for considerably less cost, according to its developers.
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