Research Associate in Molecular Biology and Mouse Models

Organization: 
Aveo Pharmaceuticals
Job Location: 
Cambridge, MA
Job Description: 

Description

The successful candidate will play an integral role in the development, generation, and manipulation of mouse embryonic stem cells to generate cancer models for use in drug development. The candidate will learn a wide array of new techniques and cutting edge technologies to assist our growing team and collaborative work environment.


Responsibilities:

  • Perform Molecular Biology techniques, such as DNA and RNA purification and analysis, DNA preparation, plasmid purification, PCR as well as transgenic construct design, transfection and subcloning to support genetic engineering of mouse cancer models
  • Support mouse models of cancer using standard mouse procedures including: embryo isolation and manipulation; small animal surgery; and mouse husbandry
  • Work on genetic manipulation of mice using the most advanced ES cell technology
  • Perform embryonic stem cell derivation, culturing, electroporation, colony picking, propagation, and freezing
  • Perform micro-injection of ES cells into blastocysts
  • Assist with genotyping of mice and with the characterization of new mouse models of cancer
Requirements: 
  • BS/BA in Life Sciences or a related Field and 2+ years of relevant laboratory experience
  • Technical expertise will be with RNA and DNA isolation and purification from cells and animal tissues
  • RNA and DNA analysis through hybridization techniques, quantitative RT-PCR, and PCR to determine genotypes, expression levels, and integrations sites
  • Experience with recombinant DNA technologies for designing and constructing vectors (plasmids and BACs) for homologous recombination and transgenic models
  • Experience with mammalian cell culture preferably embryonic stem (ES) cell culture
  • Experience in generation of mouse models through the genetic manipulation of embryonic stem cells
  • Transgenic technology such as chimera production by blastocysts injection is a plus
  • Small animal surgery/embryo transfer is a plus
Contact Information: 

Please forward resumes to Careers@aveopharma.com

AVEO is an Equal Employment Opportunity employer that values the strength diversity brings to the workplace.

Agency Notice

AVEO Pharmaceuticals does not accept unsolicited resumes from any third-party recruiters, agencies or individuals. Any such third party recruiters, agencies or individuals seeking to work with AVEO Pharmaceuticals must first contact the staffing group at careers@aveopharma.com. Only approved staffing vendors will be allowed to provide services to AVEO Pharmaceuticals. Unsolicited resumes will be deemed the property of AVEO Pharmaceuticals to follow up with as it sees applicable and no fee will be paid in the event the candidate is hired as a result of the referral or by other means. Submission of unsolicited resumes does not create any implied obligation on the part of AVEO.

About Our Organization: 

AVEO Pharmaceuticals (NASDAQ: AVEO) integrates a proprietary cancer biology platform with drug development and commercial expertise in its efforts to discover and develop targeted cancer therapeutics. The company's lead product, tivozanib, is an oral, triple VEGF receptor inhibitor with potential for a best-in-class profile. Tivozanib is currently being investigated in a global, randomized Phase 3 clinical trial called TIVO-1 comparing tivozanib to sorafenib in advanced kidney cancer, as well as additional clinical studies in other solid tumor types. AVEO's proprietary, integrated cancer biology platform offers the company a unique advantage in oncology drug development that both increases the probability of clinical success and provides a discovery engine for high-value targets. This approach has resulted in a promising pipeline of monoclonal antibodies against novel targets including HGF, ErbB3, RON, Notch and FGFR. For more information, please visit the company's website at www.aveopharma.com .