UK's MRC Funds Fourth High-Throughput Sequencing Hub in Oxford with Over $3M

MRC is funding a high-throughput sequencing center based at the Wellcome Trust Centre for Human Genetics in Oxford, adding to three other UK-based sequencing hubs that it announced this spring.

Illumina Lowers Q2 Revenue Expectations Despite Growth in Sequencing Business

Illumina said that pending stimulus grant applications caused customers to hold off on new orders during the quarter, particularly for new sequencers.

Leerink Report: About 900 Next-Gen Sequencers Deployed to Date; Market Poised for Growth

The reports note that Illumina's Genome Analyzer currently accounts for about two-thirds of next-gen sequencing systems housed at 15 international genome centers, though smaller labs tend to have a "far more equitable" split between Illumina's GA, Applied Biosystems' SOLiD, and 454 Life Sciences's GS FLX.

BC Team's Tag-Seq Protocol Reduces Costs and Improves Throughput of LongSAGE, Complements RNA-Seq

The approach modifies protocols that are used for long serial analysis of gene expression, or LongSAGE, for the Illumina Genome Analyzer and can generate two orders of magnitude more data than Sanger-based LongSAGE for considerably less cost, according to its developers.

Enabled by New Sequencing Techs, JGI Projects 300 GB of Data for 2010 Community Sequencing Program

JGI expects to generate about a third of a terabase of sequence data for CSP 2010 projects, about half on 454's sequencing platform, the other half using Illumina's technology.

Q&A: BC Cancer Agency's Robert Holt on Sequencing the Immune Repertoire

In Sequence spoke with Holt about how new sequencing technologies will soon be able to provide new insights into immunology.

Funding Update

Sequencing-Related NSF Grants Awarded June 3 — June 30, 2009

New Products

Geneious 4.7

Paired Ends

John Dausset

Consortium Seeks Sanger or 'Equivalent' for Multi-Million-Dollar Salmon Sequencing Contract

The ICSASG recommends Sanger sequencing or a technology of equivalent read length and quality, rather than one of the current next-generation sequencing technologies, for the first phase of the project. The second phase will likely involve novel sequencing technologies.

Harvard's Hybridization Capture Method Promises Flexibility, Low Cost, CNV Detection

The researchers claim that their method, published in Nature Methods this week, is less expensive and more flexible than existing array- or solution-based targeted enrichment methods. They also demonstrate that it can be used both for calling SNPs and detecting copy number variations.

NIH Awards $28.6M to Three Sequencing Centers as Part of $42M Round for Human Microbiome Project

The four-year awards go to three sequencing centers who participated in the initial phase of the project: the Washington University Genome Center, the J. Craig Venter Institute, and the Human Genome Sequencing Center at Baylor College of Medicine.

Researchers Use SOLiD for Second Analysis of African HapMap Genome Sequence, Stress Structural Variants

The Applied Biosystems-led study, which appeared online in Genome Research this week, is the second published analysis of the genome sequence of HapMap sample NA18507, a Yoruban man. Last year, a team led by Illumina published its analysis of the same sample, sequenced on Illumina's Genome Analyzer platform.