Automated RT-qPCR Utilizing the Access™ Workstation, the Echo® Liquid Handler, and RealTime Ready™ Reagents from Roche Applied Science BY Labcyte
RT-qPCR has been increasingly utilized by researchers to quantify mRNA levels. Its widespread use has been limited by an often laborious multi-step process and high reagent cost. Recent advances in non-contact reagent dispensing and next generation one step lysis reagents are enabling scientists to generate high throughput data at a much faster rate. This technical note describes the automation of three complementary tools to generate high throughout RT-qPCR data; the Echo liquid handler, RealTime Ready cell lysis reagents, and the Access workstation.
August 2, 2013
Cookie Cutter Proteolysis: Achieving Reproducible, Efficient Digestions for Proteomic Workflows BY Shimadzu
Protein sample preparation workflows for mass spectrometric analysis that involve proteolysis are often labor-intensive, time consuming, and user dependent. The introduction of variability hinders discovery initiatives. Recently, an automated protein digestion platform was developed that accelerates the digestion process while providing exceptional reproducibility. As demonstrated in this poster, it offers complete, reproducible protein digestions in as little as 30 seconds, allowing hundreds of samples to be run per day, and can be adapted to many applications.
July 24, 2013
CYP2C19 Testing: An Opportunity to Improve Patient Care BY Luminex
The cytochrome P450 class of metabolic enzymes plays an instrumental role in the metabolism and clearance of clinically prescribed drugs. Identification of patient CYP2C19 genotypes may help physicians tailor drug treatments to patients and improve a physician's ability to impact patient outcome. The Luminex xTAG CYP2C19 Kit v3 is a qualitative genotyping assay that can be used as an aid to clinicians in determining therapeutic strategy for the therapeutics metabolized by the CYP2C19 gene product, specifically *2, *3, and *17. The assay is indicated for use with the Luminex 100/200 instrument or MAGPIX with xPONENT software systems.
November 25, 2013
CYP2D6 Genotyping: An Opportunity to Improve Patient Care BY Luminex
The cytochrome P450 class of metabolic enzymes plays an instrumental role in the metabolism and clearance of clinically prescribed drugs. In particular, the Cytochrome P450 enzyme 2D6 alone is thought to be active in the enzymatic breakdown of 20-25% of all medicines prescribed including antidepressants, antipsychotics, opioids, beta-blockers, antiarrhythmics, and tamoxifen. The Luminex xTAG CYP2D6 Kit v3 assay is used to simultaneously detect and identify a panel of nucleotide variants found within the highly polymorphic CYP2D6 gene located on chromosome 22 from genomic DNA extracted from EDTA and citrate anticoagulated whole blood samples. This kit can also identify gene rearrangements associated with the deletion (*5) and duplication genotypes.
November 25, 2013
Generating Scientific Insights Through Deep Collaboration BY BioFortis
In order to make data smarter, data exploration tools need to be placed in the hands of not only the IT and informatics experts but also the scientists and domain experts that understand and can act on the data, independent of knowing how to write programming code, the structure of data or even where it is located.
The Qiagram deep collaborative environment provides just such tools, allowing researchers to explore their own data in a collaborative, transparent and effective manner.
March 1, 2013
High Throughput RT-qPCR for Gene Expression Analysis BY Labcyte
The current standard of accuracy for quantification of gene expression is reverse transcriptase quantitative PCR (RT-qPCR). A new, cost-effective sample preparation method for RT-PCR incorporates a “one-step” cell lysis buffer which eliminates the need for mRNA isolation and purification. This improved method, when combined with nanoscale non-contact liquid handling technology enables a high throughput automated workflow for gene expression.
August 2, 2013
High-throughput Miniaturized Quantitative PCR with the Echo® 525 Liquid Handler BY Labcyte
Quantitative PCR (qPCR) is a prevalent tool spanning many phases of drug discovery. Advances in qPCR detection to enable 384- and 1536-well microplate formats have incentivized researchers to miniaturize qPCR assays as a means to offset the costs of increasing throughput. To significantly reduce qPCR volumes and maintain data quality, the liquid handling methods employed for such low-volume transfers must be precise and accurate. Tipless, touchless acoustic droplet ejection with the Echo liquid handler eliminates the cost of disposable tips or tip-wash cycles and simplifies assay setup by eliminating dilution steps. This study utilized the Echo 525 liquid handler to assemble low-volume qPCR assays at speeds that keep pace with high-throughput demands. The results demonstrated the Echo 525 liquid handler effectively miniaturized reaction volumes for high-throughput qPCR in both 384- and 1536-well formats.
August 2, 2013
Infrared detection decreases nitrocellulose autofluorescence and improves RPPA assays signal‐to‐noise ratio over visible wavelength detection BY Innopsys
Advances in RPPA and more generally protein array technology have resulted in a rapidly growing market. Besides RPPAs there are also analytical and functional protein microarrays. Protein array applications in disease biomarker discovery and validation reflect the enormous potential of protein arrays in the field of personalized medicine. Until now the rise of protein array technology was limited by high fluorescence emitted by nitrocellulose. The InnoScan 710-IR infrared microarray scanner now allows an increased Signal to Noise Ratio and thus sensitivity of RPPAs by decreasing background fluorscence. In addition, with a high resolution of up to 3µm per pixel, it allows the acquisition of high quality images.
August 12, 2013
Is Your Biobank Ready for the Challenge of Biomarker-based Research? BY BioFortis
For researchers involved in biomarker-based clinical and translational research, who need to safely and effectively conduct externalized, science-driven collaborative studies, BioFortis’ Next Generation Biobanking software platform harmonizes biospecimen data with clinical and molecular data in a collaborative environment that emphasizes scientific insights, while ensuring security and compliance.
March 1, 2013
Miniaturization of the KASP™ Genotyping Assay for Maize with the Echo 525 Liquid Handler BY Labcyte
SNP genotyping of maize samples is commonly performed
in agricultural science to aide marker assisted selection, study heterosis and a variety of
biological behaviors. Its widespread adoption in agricultural science has been challenged with
increasing reagent costs and labor intensive multi-step processes. Acoustic non-contact liquid
handling using the Echo 525 liquid handler offers unique advantages to traditional processes by
incorporating a tip-less solution to deliver reagents precisely and accurately. Assay miniaturization is enabled with high accuracy and precision at volumes as low as 25nL. This study utilized the Echo 525 liquid handler to miniaturize a KASP genotyping assay for maize at a throughput meeting the demands of most high throughput production processes.
September 26, 2013
Miniaturizing siRNA Transfection Using the Echo® Liquid Handler BY Labcyte
Large-scale, high-throughput siRNA experiments require a transfer system that can facilitate reliable and reproducible transfections in high-density well-plate formats, as well as effective delivery of the siRNA molecule. This study demonstrates superior performance of the Echo liquid
handler compared to more traditional liquid handling methods for standard, reverse and lipid-free transfection of siRNA molecules into mammalian cells. Results of the study clearly demonstrate the ability of the Echo liquid handler to deliver comparable transfection efficiency, knock-down and cell morphology to reactions prepared using traditional pipeting methods. In fact, reactions prepared with the Echo liquid handler were miniaturized to use significantly less volume of siRNA and other reagents, and still yielded more representative inclusion intensities than traditional manual pipetting techniques. Such miniaturization can result in significant cost savings and greater confidence in the results from siRNA screening studies.
August 2, 2013
New Take on RADseq Enables High-Throughput Variant Discovery BY Sage Science
A new, massively parallel genotyping technique from a research team at Harvard relies on automated size selection and next-gen sequencing to boost accuracy and lower costs. Find out how the Pippin Prep from Sage Science offered reproducible, precise sizing and enabled the new method.
October 10, 2012
Oracle Health Sciences Translational Research Center: A Translational Medicine Platform to Address the Big Data Challenge BY Oracle
Oracle Health Sciences Translational Research Center is a scalable informatics solution for translational research. Its back-end data components seamlessly integrate clinical and omics data from diverse clinical data sources as well as from vendor-specific and modality-specific omics data silos, providing standardized data readily available for the front-end application.
February 4, 2013
Reducing the variability of pre-analytical handling of biological samples BY BioCision
The increasing use of biomarkers as diagnostic indicators of pathological conditions, as monitors of disease
progression or as surrogate markers of patient outcomes in clinical trials has led to a huge increase in the
number of biological samples whose accurate analysis is of vital importance...
April 26, 2012
Reverse Phase Protein Arrays for Cell Signaling: Using the InnoScan 710-IR scanner improves sensitivity and dynamic range. BY Innopsys
Reverse Phase Protein Arrays (RPPAs) are increasingly used to study cell signaling as they are highly multiplexed and allow precise quantification of protein of interests in biological samples. Such arrays promote automation, simplified work flow, and preserve sample. One of the principal challenges faced by RPPAs is to find the best combination of an array surface that will support high binding levels of viable protein probes, and a detection method that will provide high sensitivity and dynamic range. With the new InnoScan 710-IR scanner it is now possible to combine infrared detection with high sensitivity and quality images.
May 22, 2013
RNAi Screening in Primary Cells BY Lonza
Screening approaches involving either overexpression or RNAi-mediated down-regulation of genes have become a potent tool for identifying drug targets. Such screens require transfection of cells with e.g. cDNA or shRNA vectors or siRNA oligonucleotides. One important factor for screening success is the ability to efficiently transfect biologically relevant cells. Lonza’s Nucleofector™ Technology is a proven non-viral method for efficient transfection of primary cells, e.g. human T cells. The HT Nucleofector™ System, a 384-well platform, expands its application range to high-throughput transfections. With its fast plate processing time and easy integration into liquid handling systems it is a convenient tool for siRNA, shRNA or miRNA screens in biologically relevant cell types.
July 29, 2013
Say Goodbye to Manual Gels: Automated DNA Size Selection has Arrived BY Sage Science
Automated size selection is a superior alternative to manual gel extraction, allowing scientists to save time and money, improve the efficiency of sequencing runs and analysis, and perform new applications with next-gen sequencers.
December 11, 2012
Technical Note: A New Method of Snap Freezing Bacteria Using BioCision’s CoolRack System BY BioCision
The preservation of bacterial cultures by freezing has long been reported (1,2). Several protocols (3,4) recommend snap freezing in glycerol or DMSO prior to storage. One common way to snap freeze is by submerging samples in a slurry of dry ice and ethanol until frozen. BioCision’s CoolRack module...
April 26, 2012
The Clinical Performance and Value of xTAG® GPP (Gastrointestinal Pathogen Panel) BY Luminex
The first molecular diagnostic test of its kind, xTAG® GPP is a qualitative molecular multiplex test intended for the
simultaneous detection and identifcation of multiple gastrointestinal
pathogens including bacteria, viruses, and parasites from individuals
with signs and symptoms of infectious colitis or gastroenteritis. As with previous first-in-class in
vitro diagnostic (IVD) tests incorporating Luminex's xTAG chemistry, notably the
xTAG Cystic Fibrosis Kit and the xTAG Respiratory Viral Panel, xTAG® GPP was cleared through the de novo 510(k) process established by
the U.S. FDA.
September 13, 2013
Voice of the Client: CROs Role in Developing Companion Diagnostics BY Covance
In an effort to gain a better understanding of the whole companion diagnostics arena, we surveyed experts from leading biopharma and in vitro diagnostic companies on how they were currently implementing their companion diagnostics strategies. We enquired as to their general needs around the companion diagnostic space, their strategy for building internal capabilities versus engaging external collaborators, and ultimately asked them what role, if any, they felt CROs could play in their drug/companion diagnostic co-development strategy. This paper reports on the survey.
August 22, 2013