An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture BY Bioo Scientific
Targeted sequencing is an important tool in analyzing disease or exome mutations. In this study, we describe how Bioo Scientific’s NEXTflex™ DNA Pre-Capture Combo Library construction kits were used in conjunction with NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples. We identified 96-98% of known HapMap SNPs in the NimbleGen SeqCap EZ Exome v3.0 (64 Mb) and SeqCap EZ Design – Comprehensive Cancer Design (3.9 Mb) probes with a high percentage of reads mapping to the targeted regions. This study illustrates both the ability of Bioo Scientific’s NEXTflex library construction kit to produce high-quality material for NGS, and the robust performance of the NimbleGen Sequence Capture technology.
May 7, 2013
BioXM Knowledge Management Environment BY Biomax
The BioXM™ Knowledge Management Environment is a fully customizable knowledge management (KM) solution for better integrated and knowledge-based drug discovery and development. This project-centered, distributed software platform provides a central inventory of information and knowledge. Users create, manage and visualize scientific models as an extendible network of interrelated concepts. The BioXM platform facilitates communication and collaboration within research environments, allowing you to focus on science.
August 13, 2012
Electromyography Study of Pipetting Ergonomics and the Correlation to Reducing Repetitive Stress Disorders with Biotix FlexFit BY Biotix
The repetition of standard laboratory techniques, especially pipetting, increases the risk of Repetitive Stress Disorder (RSD) for bench scientists. In a study published by Bjorksten et al., manual pipetting for little more than 300 hours per year increases the risk of hand and shoulder injuries...
May 16, 2012
ePathology Improves Data Accuracy & Study Productivity! – Amicus Study Confirms BY Leica Biosystems
With current glass slide methods, sharing and scoring slides within a study from one pathologist to another at a different organization or site, meant that slides had to be packed, shipped and tracked. Often the reading pathologist was required to follow the slides to their location, losing time and increasing study costs. This whitepaper compares conventional microscopy versus ePathology and the impact of reader accuracy and productivity during a GLP clinical study.
April 12, 2013
Generating Scientific Insights Through Deep Collaboration BY BioFortis
In order to make data smarter, data exploration tools need to be placed in the hands of not only the IT and informatics experts but also the scientists and domain experts that understand and can act on the data, independent of knowing how to write programming code, the structure of data or even where it is located.
The Qiagram deep collaborative environment provides just such tools, allowing researchers to explore their own data in a collaborative, transparent and effective manner.
March 1, 2013
Impacting Clinical Outcomes: Computational Analysis of Complex Genomics Data Sets BY Covance Genomics Lab
Data analysis, interpretation, and integration of high volumes of multidimensional data are the key to elucidating the mechanism of action of pharmaceutical compounds, understanding the pathways to disease onset and progression, biomarker and target identification. In today's era of large data sets, biopharmaceutical companies are utilizing computational biology to comb through massive sets of genomic data to find links between specific genotypes and diseases, screen drug data to identify therapeutic candidates, as well as identify responders to treatment.
April 16, 2013
Is Your Biobank Ready for the Challenge of Biomarker-based Research? BY BioFortis
For researchers involved in biomarker-based clinical and translational research, who need to safely and effectively conduct externalized, science-driven collaborative studies, BioFortis’ Next Generation Biobanking software platform harmonizes biospecimen data with clinical and molecular data in a collaborative environment that emphasizes scientific insights, while ensuring security and compliance.
March 1, 2013
Molecular indexing for improved RNA-Seq analysis BY Bioo Scientific
In this application note, Bioo Scienti.c Corporation and Cellular Research introduce a new kit for high precision gene expression analysis by RNA-Seq. We demonstrate that the NEXTflex™ qRNA-Seq™ Kit can be directly substituted into paired-end library preparation without affecting conventional RNA-Seq analysis methods. This new kit efficiently generates libraries equivalent to standard RNA-Seq libraries with sample barcodes, but with the added feature of molecular indexing. The value of using the added molecular indexing feature depends upon experimental design, data objectives, and sequencing depth for a given library complexity.
May 22, 2013
New Take on RADseq Enables High-Throughput Variant Discovery BY Sage Science
A new, massively parallel genotyping technique from a research team at Harvard relies on automated size selection and next-gen sequencing to boost accuracy and lower costs. Find out how the Pippin Prep from Sage Science offered reproducible, precise sizing and enabled the new method.
October 10, 2012
Oracle Health Sciences Translational Research Center: A Translational Medicine Platform to Address the Big Data Challenge BY Oracle
Oracle Health Sciences Translational Research Center is a scalable informatics solution for translational research. Its back-end data components seamlessly integrate clinical and omics data from diverse clinical data sources as well as from vendor-specific and modality-specific omics data silos, providing standardized data readily available for the front-end application.
February 4, 2013
Reducing the variability of pre-analytical handling of biological samples BY BioCision
The increasing use of biomarkers as diagnostic indicators of pathological conditions, as monitors of disease
progression or as surrogate markers of patient outcomes in clinical trials has led to a huge increase in the
number of biological samples whose accurate analysis is of vital importance...
April 26, 2012
Reverse Phase Protein Arrays for Cell Signaling: Using the InnoScan 710-IR scanner improves sensitivity and dynamic range. BY Innopsys
Reverse Phase Protein Arrays (RPPAs) are increasingly used to study cell signaling as they are highly multiplexed and allow precise quantification of protein of interests in biological samples. Such arrays promote automation, simplified work flow, and preserve sample. One of the principal challenges faced by RPPAs is to find the best combination of an array surface that will support high binding levels of viable protein probes, and a detection method that will provide high sensitivity and dynamic range. With the new InnoScan 710-IR scanner it is now possible to combine infrared detection with high sensitivity and quality images.
May 22, 2013
Say Goodbye to Manual Gels: Automated DNA Size Selection has Arrived BY Sage Science
Automated size selection is a superior alternative to manual gel extraction, allowing scientists to save time and money, improve the efficiency of sequencing runs and analysis, and perform new applications with next-gen sequencers.
December 11, 2012
Screening DNA Sequences Against a Protein Database Using a Hybrid-Core Implementation of Smith-Waterman BY Convey Computer
This paper discusses SWSearch, a search and alignment program based on the Smith-Waterman algorithm that is optimized for Convey's hybrid core architecture to dramatically reduce the time required to perform large numbers of local alignments. Results show that SWSearch on Convey's HC-2ex runs up to 15 times faster than the Smith-Waterman algorithm in SSEARCH on a commodity x86 system.
July 12, 2012
Security and Protection with Industry’s First Aerosol Barrier Pipette Tip with Color-Change Visual Alert BY Biotix
The use of aerosol barrier (filter) pipette tips has become best practice in protocols utilizing PCR amplification and for laboratories performing molecular assays in research and in the clinic. This white paper details a scientific study of Biotix pipette tips with Delta Filter technology. The study tested both aerosol blocking efficiency and filter inhibition and enhancement. Test data demonstrated the proven ability of Delta Filter to block aerosols as effectively as competitive tips. The challenge methods, covering viruses to nanoparticles, also showed that the Delta Filter colorant does not cause inhibition or enhancement and is suitable for use with sensitive bioassays.
May 22, 2012
Setting a New Standard in Sample Delivery BY Biotix
The high cost of rare biological samples, coupled with the increasing sensitivity of today’s molecular methods, demands high precision pipetting by laboratory technicians. This study evaluates sample loss of leading pipette tips in comparison to Biotix pipette tips with X-Resin. Efficiency of sample delivery was measured among three sample types; DNA, proteins, and nanoparticles. The study determined that Biotix tips with X-Resin provide superior performance in sample delivery across a broad range of sample types, and are ideal for high sensitivity assays.
May 22, 2012
Technical Note: A New Method of Snap Freezing Bacteria Using BioCision’s CoolRack System BY BioCision
The preservation of bacterial cultures by freezing has long been reported (1,2). Several protocols (3,4) recommend snap freezing in glycerol or DMSO prior to storage. One common way to snap freeze is by submerging samples in a slurry of dry ice and ethanol until frozen. BioCision’s CoolRack module...
April 26, 2012