Women Wanted

January 04, 2012

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At US News & World Report's STEM Education blog, the University of Virginia's Joanne McGrath Cohoon says that technical fields need capable women, "especially at a time when unemployment is high and our economy is weak." But McGrath Cohoon says attracting women to work in STEM fields will be difficult until "women experience the same conditions as their male classmates," and gender-based stereotypes fade.

"Evidence continues to mount that capable women in technical fields have less confidence than men that they will be successful," McGrath Cohoon says. "Unfortunately, many of these women are wrong. They could succeed in engineering or computing, and it is in our interest that they do."

In the meantime, she adds, educators can take action by giving their female students "lots of opportunities to succeed at technical tasks." McGrath Cohoon also suggests verbal encouragement. "With these steps, we could double the number of technical people working to make this a better world," she says.

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Advances in Single-Cell Genomics: Live Cell RNA and Circulating miRNA Detection

Sponsor: EMD Millipore

Data presented in this webinar illustrates the value of live cell analysis at the single-cell level to identify differences in expression levels across populations of cells. The cells remain intact for downstream analysis. Our experts also discuss the use of SmartFlare RNA detection probes for the direct quantification of circulating miRNAs with rapid processing of blood plasma/serum, which is done without the use of enzymes. Using circulating miRNAs with established roles in cancer and quality control, we can accurately detect these miRNAs in plasma using a microplate fluorometer within an hour after plasma preparation.

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Optimization of NGS Library Preparation: Low Inputs and Fast, Streamlined Workflows

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Available here.
Young Investigator Profile

Andrew Johnson

Investigator
National Heart, Lung, and Blood Institute

A Bridge Across the Gap

There can be a bit of a chasm between dry lab work and wet lab work. But NHLBI's Andrew Johnson aims to close that gap. "A lot of things that I attempt in terms of projects try to bridge those barriers between the dry lab, computational side of things and wet lab experiments that are two different worlds," he says.

Currently, Johnson's work focuses on developing tools. During the early days of genome-wide association studies, he noticed that it was difficult for researchers to compare results generated across various array platforms. In conjunction with scientists at the Broad Institute, he developed a tool called SNAP to do just that. "[It] was pretty successful in terms of allowing people to rapidly query for proxy markers, but also just to do basic annotation of SNPs, like chromosome position, gene, and also to do plotting for regional genetic association plots," he says.
Blog

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A contributor to a post-publication review site notes possible issues with a recent stem cell paper.

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Use it Well

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May 23, 2013

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The University of Würzburg's Cynthia Sharma and colleagues undertook a transcriptomics-based analysis of the gastroenteritis-causing bacterial species Campylobacter jejuni. The team used its so-called differential RNA sequencing strategy to sequence and compare the transcriptomes of four C. jejuni isolates (three from humans and one from a chicken), applying a new method to automatically annotate transcription start sites in each. "Overall," they write, "our study provides new insights into strain-specific transcriptome organization and [small RNAs], and reveals genes that could modulate phenotypic variation among strains despite high conservation at the DNA level."

Mosaic genome structure of the barley powdery mildew pathogen and conservation of transcriptional programs in divergent hosts
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The barley powdery mildew (Blumeria graminis f. sp. hordei) pathogen genome is comprised of chunks of sequence that are particularly rich or replete in polymorphisms, according to a study by researchers from the Max Planck Institute for Plant Breeding Research. The team sequenced the genomes of two Bgh isolates from Europe, comparing each to the barley powdery mildew reference genome. The newly sequenced isolates each contained distinct combinations of sequence blocks with high or low SNP concentrations — isolate-specific mosaic genomes that point to "exceptionally large standing genetic variation in the Bgh population," study authors say. Meanwhile, their transcriptome sequencing experiments offered a look at genes used by Bgh during attempted infiltration of barley or immunocompromised Arabidopsis.
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Kevin Hrusovsky is resigning his post at PerkinElmer as senior VP and president of the Life Science and Technology division. Hrusovsky will serve as a consultant to the company for up to one year, beginning in June. He joined PerkinElmer through the company's acquisition of Caliper Life Sciences, where he was CEO and president.

Hologic has appointed former Beckman Coulter head Scott Garrett to its board of directors, where he will serve on the corporate development committee.
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Gina Costa is now senior director of genomic applications at Illumina. She joins Illumina from Life Technologies, where she was senior director of genetic analysis, working on development of the Ion Torrent and SOLiD sequencing technologies. She has also held positions at Agencourt Bioscience and Roche's 454 Life Sciences.

Bioinformatics firm Golden Helix has hired Andreas Scherer to be its new president and CEO. Scherer has managed large global software services businesses, and he started his executive career at AOL/Netscape. He will replace Former CEO Christophe Lambert, who will take on the new role of company chairman.
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2013 ASCO Annual Meeting: Building Bridges to Conquer Cancer
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Jun 8-11 / Paris
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International Meeting on Cell-Free DNA
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Science
An international team has sequenced the genome of the carnivorous bladderwort plant, Utricularia gibba. Their findings suggest that the carnivorous plant has ditched virtually all its non-coding DNA, retaining a set of sequences that's almost exclusively genic. "What that says is that you can have a perfectly good multicellular plant with lots of different cells, organs, tissue types and flowers, and you can do it without the ['junk' DNA]," said co-corresponding author Victor Albert.
Business
Agilent Technologies announced a restructuring program expected to reduce its headcount by about 450 employees and save the company $50 million annually in operating expenses. CEO Bill Sullivan said that the focus of the restructuring will be on Agilent's Electronic Measurement Group and that the company will explore opportunities "to streamline our organization around the world." The firm also announced that its Q2 revenues were flat year over year.
Funding
The US Department of Energy's Joint Genome Institute has funded six new initiatives to develop technologies that will help JGI and its users conduct their research efforts into microbiology, metagenomics, and plant genomics. The projects will be supported under the Emerging Technologies Opportunity Program with a total of around $3.5 million over the next two years. Among the researchers receiving funding are Stephen Quake and Jay Shendure.
GenomeWebinars
GenomeWebinar: Advances in Single-Cell Genomics: Live Cell RNA and Circulating miRNA Detection

Sponsor: EMD Millipore

GenomeWeb and EMD Millipore invite you to view an archived webinar discussing new approaches to detect RNA at the single-cell level as well as new probes for the direct quantification of circulating miRNAs. In this free online seminar, recorded April 25, 2013, our expert panel shares protocols for improved RNA and miRNA detection.

On-demand recording available here.

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