Scientists at Konkuk University in Seoul this week present a microarray-based diagnostic method for chronic myelogenous leukemia genotyping in The Journal of Molecular Diagnostics. In a paper published online in advance, the team describes its approach, which integrates multiplex reverse-transcription, asymmetric PCR and hybridization, "all in the same mixture in a chamber assembled on the surface of capture oligonucleotide probes immobilized on a glass slide." The team successfully identified four predominant types of chimerical BCR-ABL1 RNA using this integrated system. Among other things, the authors write, this approach minimizes the number of steps required "for automated detection of different types of chimerical CML [chronic myelogenous leukemia] RNA."
In another paper published online in advance this week, researchers at Rosetta Genomics present a classification of the four main lung cancer types using an miRNA-based diagnostic assay. This assay is an extension of Rosetta's miRview squamous test, developed to differentiate squamous from non-squamous non-small cell lung cancers. The firm's new miRview lung assay can differentiate between squamous cell carcinoma, non-squamous non–small cell lung cancer, carcinoid, and small cell carcinoma. Writing in The Journal of Molecular Diagnostics, the Rosetta researchers say that "miRview lung is a simple and reliable diagnostic assay that offers an accurate and standardized classification tool for primary lung cancer using pathologic and cytologic samples."
The Sample has more on miRview here.